Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116

October 2014 Vol. 3(8), pp 119-126

Copyright © 2014 Global Advanced Research Journals


Full Length Research Paper

Extracellular bio-synthesis, characterization and morphology of silver nanoparticles

1,2E.H. El- Mossalamy, 1Abdullah Yousif Obaid, 1L. M. Al-Harbi 3N.F.Al Harby 4 A. A. Al-Owais and 5Zaheer Khan


1Department of Chemistry, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia

2Chemistry Department, Faculty of Science, Benha University, Benha Egypt

3Chemistry Department, Faculty of Science and Arts, Al Qassim University.

Saudi   Arabia

4Chemistry Department, King Saud University, P.O.Box 272047, Riyadh-11352, Saudi Arabia

5Department of Chemistry, Jamia Millia Islamia (Central University), New Delhi-110025, India

*Corresponding Author's Email:;


Accepted 15 October, 2014




The effect of cetyltrimethylammonium bromide, CTAB has been studied on the optical properties and morphology of advanced Ag-nanoparticles (AgNPs) usingOriental plane leaves extract as a reducing-, stabilizing- and  capping-agent for the first time.  The formation of AgNPs  was monitored by measuring the UV-vis. spectra at different time intervals (5, 10, 20 and 30 min) after adding the leaves extract (from 1 to 5 cm3) to the different AgNO3 solutions ([Ag+] = 4.0 , 8.0, 12.0 , 16.0 × 10-4 mol dm-3 ). The sigmoidal nature of the reaction- time plots suggests the involvements of an autocatalytic reaction path. In presence of CTAB, the peak at 450 nm is shifted to shorter wavelength, i.e., 425 nm and sharpness of the surface resonance plasmon (SRP) band also decreases. The results confirm a significant change in the morphology and/or agglomeration tendency with CTAB.  Transmission electron microscopy (TEM) results show the formation of stable AgNPs at different concentration of AgNOgives mostly spherical particles with diameter ranging from 10 to 30 nm.

Keywords: Oriental plane; Silver particles; CTAB; Morphology.



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