Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
January 2014 Vol. 3(1), pp. 026-032
Copyright © 2014 Global Advanced Research Journals


Full Length Research Paper

Synergistic effect of combined extract of Bryophyllum pinnatum and Aloe barbadensis enhances anti-microbial activity in-vitro

Abdulazeez A. Abubakar1, Hassan A. Oladele2* Adewole A. Adejumoke2, Fadairo J. Kayode2

1Department of Biosciences and Biotechnology Kwara State University, Malete- Ilorin  Nigeria.
2Medical Laboratory Sciences Department, Achievers University, Owo Ondo State, Nigeria

*Corresponding Author E-mail:,; Tel: +2348034538409, +2348053283469

Accepted 21 January, 2014


This study was conducted between June and December 2012 in Achievers University, Owo-Nigeria to determine in-vitro antimicrobial activity of combined extract of Bryophyllum pinnatum and Aloe barbadensis on some clinical bacterial isolates and control organisms. The leaves from both herbs were collected, washed, grinded, filtered and the filtrates dried, weighed re-dried and re-weighed again until the weights were constant. Dried filtrate of each extract was grinded with pestle and mortar into powdery form. One gramme of each powdery extract was dissolved in ten milliliters of sterile distilled water to produce 100mg/ml. Bryophyllum pinnatum extract and Aloe barbadensis extract were mixed together at ratio of 6:4 to prepare various concentrations (10-1 to 10-9 mg/ml). of admixed extract. Each prepared concentration was tested on different bacteria isolates and control organisms using punch-hole antimicrobial sensitivity method .Findings  from this study showed that a billionth concentration (mg/ml) of the mixed extract inhibited the growth of all common clinical isolates examined except Pseudomonas aeruginosa and Klebsiella Pneumoniae that were inhibited at high concentration (10-3) of the extract. Assessing  the efficacy of the fresh and stored extracts, the results showed no significant difference in the zones of inhibition of the bacteria isolates with respect to varying concentrations of fresh and stored extracts (P>0.05). By comparing the activity of the extract on the test isolates and control organisms there was insignificant variation in the zones of inhibition produced by the organisms (P>0.05). In conclusion, the synergism between the two herbs increased their antimicrobial potentials as a result of  positive interaction emanating from their combination.

Keywords: Synergism, Bryophyllum pinnatum, Aloe barbadensis, anti-bacterial

Related Articles

Original Research Articles
Wafaa El-komy1, Soheir Mahmoud1, Hoda Sabry1, Soheir Mansy2 and Nevine Guirguis1
Changes in parasitic load, phagocytosis and ultra-structural pattern in experimental cryptosporidiosis following combined (Antox and Nitazode) treatment
Glo. Adv. Res. J. Med. Med. Sci. March 2016 Vol: 5(3): - [Abstract] [Full Text - PDF] (3,544 KB)
Sami S Ashgar, Barhameen AA Abeer, Ayman Johargy, Hamdi M El-Said, Mohammed H Mukhtar and Abdullah A Saati
Prevalence of Streptococcus pyogenes Among Pre-School Children Ages 4 to 6 in Makah city, Saudi Arabia
Glo. Adv. Res. J. Med. Med. Sci. January 2017 Vol: 6(1): - [Abstract] [Full Text - PDF] (111 KB)
Tinatin Doolotkeldieva, Saykal Bobusheva and Maxabat Konurbaeva
Impact of Biocontrol Agent Streptomyces fumanus on Bacterial Communities in the Rhizosphere of Wheat and Soybean in Newly Cultivated Soil
Glo. Adv. Res. J. Med. Med. Sci. April 2015 Vol: 4(4): - [Abstract] [Full Text - PDF] (521 KB)
Hind A. A. Al-Zahrani
Genetic mutagenesis through Transposable element 5 (Tn5) to improve beta-D-galactosidase productivity from different bacterial strains
Glo. Adv. Res. J. Med. Med. Sci. May 2018 Vol: 7(3): - [Abstract] [Full Text - PDF] (1,393 KB)
Andy Ramu and Veluchamy Vijayakumar
Antidiabetic activity of 7-methoxycoumarin from the bark of Marine plant Rhizophora mucronata
Glo. Adv. Res. J. Med. Med. Sci. May 2016 Vol: 4(1): - [Abstract] [Full Text - PDF] (613 KB)
Engy Yousry El sayed, Eman Mohamoud Ezzat, Rania Ali Amr
Emergence of gram positive bacteria as a causal agents of spontaneous bacterial peritonitis.
Glo. Adv. Res. J. Med. Med. Sci. May 2014 Vol: 3(4): - [Abstract] [Full Text - PDF] (130 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Abdulazeez A Abubakar on Google Scholar
Abdulazeez A Abubakar on Pubmed
Hassan A Oladele on Google Scholar
Hassan A Oladele on Pubmed
Adewole A Adejumoke on Google Scholar
Adewole A Adejumoke on Pubmed
Fadairo J Kayode on Google Scholar
Fadairo J Kayode on Pubmed


Viewed 2045
Printed 512
Downloaded 1831
Powered By iPortal Works