Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
October 2017 Vol. 6(10), pp. 240-244
Copyright © 2017 Global Advanced Research Journals

 

Full Length Research Paper 

Antibacterial activity of crude herbal extracts of Zingiber officinale and Curcuma longa against 13 reference bacterial species

Sami S. Ashgar

Microbiology Department, College of Medicine, Umm Al-Qura University, Saudi Arabia.

E-mail: ssashgar70@hotmail.com; Mobile: +966(0) 505 794 955

Accepted 09 October, 2017 

Abstract

Medicinal plants acquired great attention for their miracle properties against various human illnesses. In this laboratory-based study, Antibacterial effectiveness of Curcuma longa and Zingiber officinale (Ginger) were extracted in aqueous, 70% ethanol and ethyl acetate solvents and investigated against 13 various American type culture collection (ATCC( strains  by the mean of determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Results demonstrated that ethyl acetate extract of Curcuma longa was superior to other solvents versus Enterococcus faecalis, Enterococcus faecalis (VRE), Staphylococcus aureus (MRSA) and Staphylococcus epidermidis with inhibition zones range (12-19mm) and exhibited killing concentration varies between 25-50 mg/ml. Acinetobacter baumannii expressed visible sensitivity to Curcuma longa extracted in aqueous, ethanol and ethyl acetate solvents. Bactericidal activity was experimentally constant at a concentration of 50mg/ml. Ethyl acetate extract of Ginger, on the other hand, was the only extract that showed antibacterial activity against three reference bacterial strains include E. faecalis, S. aureus and Staphylococcus aureus (MRSA), with inhibition zones range (12-19mm) and MBC value of 100, 12.5 and 25 mg/ml, respectively. In conclusion, demonstrated antibacterial activity of Curcuma longa and Zingiber officinale can be further invested as potential natural antibiotics against some multi-resistant pathogens. 

Keywords: Curcuma longa, Zingiber officinale, MIC, MBC, ethyl acetate, ethanol, aqueous, ATCC strains.

 

References

Akintobi OA, Onoh CC, Ogele JO, Idowu AA, Ojo OV, Okonko IO (2013). Antimicrobial activity of Zingiber officinale (ginger) extract against some selected pathogenic bacteria. Nature and science. 11(1): 7-15.

Al-Daihan S, Al-Faham M, Al-shawi N, Almayman R, Brnawi A, Zargar S, Bhat RS (2013). Antibacterial activity and phytochemical screening of some medicinal plants commonly used in Saudi Arabia against selected pathogenic microorganisms. J. King Saud Uni. Sci. 25(2): 115-120.

Balunas MJ, Kinghorn AD (2005). Drug discovery from medicinal plants. Life Sciences. 78(5): 431-441.

Bibi Y, Nisa S, Chaudhary FM, Zia M (2011). Antibacterial activity of some selected medicinal plants of Pakistan. BMC Complement Altern Med. 11: 52.

Chakraborty B, Nath A, Saikia H, Sengupta M (2014). Bactericidal activity of selected medicinal plants against multidrug resistant bacterial strains from clinical isolates. Asian Pacific J. Trop. Med. 7(Supplement 1): S435-S441.

Chauhan P, Keni K, Patel, R (2017). Investigation of phytochemical screening and antimicrobial activity of Curcuma longa, 4, 153-163 pp.

CLSI (1998). Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guidelin. CLSI document M26-A [ISBN 1-56238-384-1].

CLSI (2015). Methods for dilution Antimicrobial Susceptibility Test for Bacteria that Grow Aerobically, Approved Standard. CLSI document M07-A10 (10th ed.).

Indrawati  IDA (2017). Antibacterial activity of ethanolic extracts of rhizome from three ginger varieties against acne isolated bacteria, 9.

Kocaadam B, Şanlier, N (2017). Curcumin, an active component of turmeric (Curcuma longa), and its effects on health. Critical Reviews in Food Science and Nutrition, 57(13): 2889-2895.

Nikhil Singh SG, Vaibhav Rathore (2017). Comparative Antimicrobial Study of Ethanolic Extract of Leaf and Rhizome of Cuucuma longa Linn. Pharmacogn J. 9(2): 208-212.

Tambe A, Deokar S, Pawar R (2016). Evaluation of Antimicrobial Activity and Phytochemical Analysis of Zingiber officinale (Ginger) Rhizome Extract. Int. J. Allied Practice, Res. Rev. 3(6).

Teow SY, Liew K, Ali SA, Khoo AS, Peh SC (2016). Antibacterial Action of Curcumin against Staphylococcus aureus: A Brief Review. J. Trop. Med. 2016: 2853045.

Vu TT, Kim H, Tran VK, Le Dang Q, Nguyen HT, Kim H, Kim IS, Choi GJ, Kim JC (2016). In vitro antibacterial activity of selected medicinal plants traditionally used in Vietnam against human pathogenic bacteria. BMC Complement Altern. Med. 16: 32.

WHO (2000). General guidelines for methodologies on research and evaluation of traditional medicine. p. 1-71.

WHO (2002). WHO Traditional medicine strategy 2002-2005.

WHO (2017). Antibacterial agents in clinical development: an analysis of the antibacterial clinical development pipeline, including tuberculosis., Geneva.


Related Articles


Original Research Articles
Eduardo Scott Fragoso Cerqueira, Marina Emanoella Seruti Pelógia, Camila Pinho Balthazar Silveira, Alisson Senna Fechis, Thiago André Salvitti de Sá Rocha, José Luiz Laus and Fabrício Singaretti de Oliveira
Suture analysis and arterial traction test in dogs fixed on alcohol and preserved on saline solution aiming surgical practice
Glo. Adv. Res. J. Med. Med. Sci. November 2017 Vol: 6(11): - [Abstract] [Full Text - PDF] (94 KB)
Magdy A Abu-Gharbia, El-Sayed M Soltan, Osman M El-Maghraby and Walaa M Abd El-Raheem
Antibacterial effect of some Egyptian plants on antibiotic-resistant oral bacteria
Glo. Adv. Res. J. Med. Med. Sci. January 2015 Vol: 4(1): - [Abstract] [Full Text - PDF] (121 KB)
Mohamed Abd El Rehim A. Hassan
Pedological studies for some soil on sides of the Ismailia Canal in the Eastern part of Nile Delta in Egypt
Glo. Adv. Res. J. Med. Med. Sci. August 2017 Vol: 6(8): - [Abstract] [Full Text - PDF] (3,666 KB)
Nagwa Thabet Elsharawy
The Inhibition Effect of Thyme and Coriander Essential Oils on of Campylobacter Jejuni and Some of Their Virulence Genes on Chicken Burger and Chicken Shawerma Products
Glo. Adv. Res. J. Med. Med. Sci. August 2018 Vol: 7(6): - [Abstract] [Full Text - PDF] (1,879 KB)
Naksheen Merza Mahmood, Nadia Farid Ramadan, Shadia Mohamed Hassan, HodaYaaqob Sabry and Mona Mohamed Magdy
Therapeutic effect of Phoenix dactylifera against cryptosporidiosis in immunocompromised mice
Glo. Adv. Res. J. Med. Med. Sci. March 2016 Vol: 5(3): - [Abstract] [Full Text - PDF] (3,707 KB)
Sambo S1 Faruk UZ2 and Shahida AA3
Ethanol Production from Fresh and Dry Water Hyacinth Using Ruminant Microorganisms and Ethanol Producers
Glo. Adv. Res. J. Med. Med. Sci. August 2015 Vol: 4(1): - [Abstract] [Full Text - PDF] (461 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Sami S Ashgar on Google Scholar
Sami S Ashgar on Pubmed

Statistics

Viewed 1028
Printed 134
Downloaded 995
Powered By iPortal Works