Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
October 2017 Vol. 6(10), pp. 249-256
Copyright © 2017 Global Advanced Research Journals
Full Length Research Paper
Effects of the age and sex of an automated assay for adenosine deaminase among a Brazilian population sample with pleural tuberculosis
Joeber Bernardo Soares de Souza1, Cyro Teixeira da Silva Junior2*, Jorge Luiz Barillo1, Patricia Siqueira Silva3, Salim Kanaan4, Elizabeth Giestal de Araujo4 and Analucia Rampazzo Xavier5
1MD, Physicians, Hospital Universitário Antônio Pedro, Fluminense Federal University, Niteroi, State of Rio de Janeiro, Brazil.
2PhD, MD, Associate Professor, Department of Clinics, School of Medicine, Fluminense Federal University, Niteroi, State of Rio de Janeiro, Brazil.
3Bsc, Biologist, Clinical Analysis Posgraduate Program, Fluminense Federal University, Niteroi, State of Rio de Janeiro, Brazil.
4PhD, MD, Associate Professor, Department of Neurobiology, Fluminense Federal University, Niteroi, State of Rio de Janeiro, Brazil.
5PhD, Associate Professor, Department of Clinical Pathology, School of Medicine, Fluminense Federal University, Niteroi, State of Rio de Janeiro, Brazil.
*Corresponding Author E-mail: firstname.lastname@example.org
Accepted 26 October, 2017
Adenosine deaminase (ADA) is a diagnostic biomarker assay for pleural tuberculosis (P-TB) using pleural fluid (P-ADA). Our aim was to establish a new reference value for P-ADA in consideration of the influence of age and sex on the cut-off value for pleural TB in a Brazilian population sample. Diagnostic accuracy study conducted in a Center for Teaching and Research in Pleurology at a hospital of a public university. An automated ADA assay was performed using a commercial kit. In the P-TB group (n=26), 11 patients were male (42%) and 15 patients were female (58%). The median ages of the patients in the P-TB, non-P-TB (n=95), and overall pleural effusion groups (n=121) were 46.0±18.98, 64.0±17.88, and 62.0±19.14 years, respectively. A correlation of the P-ADA level with sex in patients with P-TB was not found to be significant (rho= -0.191, P=0.5739). However, when the P-ADA levels were dichotomized by the median age (62 years) the Spearman’s correlation was negative and non-significant in all patients (rs= -0.0061, P=0.9630), tuberculosis (rs=-0.0857, P=0.9194), and non-tuberculosis groups (rs= -0.2789, P=0.0813). For the diagnosis of pleural TB, the best cut-off value established according to the ROC curve for P-ADA was ≥31.5 IU/L (AUC=88.2, P=0.0001). Age and sex did not have a significant influence on the clinical decision limit for the diagnosis of TB pleural effusion using the ADA automated assay. For the diagnosis of pleural TB, the cut-off value established for P-ADA was ≥31.5 IU/L.
Keywords: Adenosine deaminase, reference values, pleural effusion, pleural tuberculosis, tuberculous pleurisy
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