Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
October 2018, Vol. 7(8), pp. 165-168
Copyright © 2018 Global Advanced Research Journals

 

Full Length Research Article

Protection by Dialium indium (Fruit) against Pb2+-Induced Microcytic Anemia and Chromosome Aberration in Albino Rats In Vivo

Tugbobo O.S.*, Abegunde S.M. and  Idowu K.S.

Department of Science Technology, Federal Polytechnic, Ado-Ekiti, Nigeria

*Corresponding Author E-mail: tugbobooladimeji@yahoo.com 

Accepted 24 October, 2018 

Abstract

The chelating ability and anticlastogenic potential of Dialium indium aqueous extract on lead-induced microcytic anemia and chromosome aberration was investigated in albino rats. Twenty rats were randomly grouped into four where rats in group B were treated daily with oral administration of 2.5mg/kg lead acetate, animals in C were fed simultaneously with 2.5mg/kg lead acetate and 200mg/kg Dialium indium extract while group D animals were fed 200mg/kg Dialium indium extract only and animals in group A served as control. The animals were sacrificed after four weeks of exposure and the toxicological effect of lead and the plant extract on hemoglobin-enzymes and chromosomes were equally assessed via micronucleus assay. The results from hematogical and micronucleus assay suggest that lead could possibly induce microcytic-anemia and as well initiate the development of chromosome aberration in albino rats as observed in group B animals, while the extract offered protective effect to animals in group C. However, the extract anticlastogenic and chelating potential was in synergy with the activity of hemoglobin-enzymes as the frequency of chromosome aberration was significantly (P<0.05) low in group D animals compared to those in group B. 

Keywords: Microcytic anemia, Dialium indium, lead acetate, anticlastogenic, hemoglobin-enzymes.

 

References

Aguawa CN, Mittal GC (1981). Study of antiulcer activity of aqueous extract of leaves of Pyrenacanthia staudtii using various models of experiment gastric ulcer in rats. Eur. J. Pharmacol. 74: 215-220.

Das T, Roychoudhury A, Sharma A, Talukder G (1993). Modification of cytotoxic effect of arsenic by crude extract of garlic in mice. Inter. J. Pharmacognosy, 31: 316-320.

Fejes S, Blazovics A, Lugasi A, Lemberkovics E, Petri G, Kery A (2000). In vitro antioxidant activity of Anthriscus cerefolium (Hoffin) extracts. J. Ethnopharmacol. 69: 259-265.

Humphrey DI (1991). Effect of exposure to excessive quantity of lead on animals. Br. Vet. J, 147: 18-30.

Leonard A (1991). Heavy metals inhibitory effect on thiols-enzymatic groups. Brit. Med. J. 203-208.

Preston RJ, Dean BJ, Gallowxy S, Holden H, Mcfee AF, Shlby M (1987). Mammalian in vivo cytogenetic assay analysis of chromosome aberration in bone marrow cells. Mutat. Res. 189: 157-165.

Rimbach G, Hohler D, Fischer A, Roy S (1999). Methods used to access free radicals and oxidative stress in biological system. Arch. Tiereinahr. 53: 203-222.

Semiz A, Sen A (2007). Antioxidant and chemoprotective properties of bitter melon fruit extract. Afri. J. Biotechnol. 6: 273-277.

Sharma A, Talukder G (1987). Effect of metals on chromosomes of higher organisms. Environ. Mutagen. 9: 191-226.

Sharma AK, Sharma A (1994). Chromosome techniques; manual of Hardwood Publishers, pp.368, Switzerland.

Sofidiya MO, Odukoya OA, Familoni OB, Inya-Agha ST (2006). Free radicals scavenging activity of some Nigerian medicinal plant extracts. Park. J. Biol. Sci. 9: 1438-1441.

Sokal RR, Rohlf FI (1987). Single classification of analysis of variance. Introduction to Biostatistics pp. 363 W.H. Freeman San Francisco.

Stohs ST, Bagchi D (1995). Free radicals, Biol. Med. 18: 321-336.

Tugbobo OS, Oloyede OI, Adewale  OB (2014).Dietary protection by garlic against lead-induced oxidative stress.and genetic birth defects. Oxid. Antioxid. Med. Sci. 3 (1): 79-82


Related Articles


Original Research Articles
Imam AA Mekkawy, Usama M Mahmoud and Rehab H Mohammed
Protective effects of tomato paste and Vitamin E on Atrazine-Induced Hematological and biochemical characteristics of Clarias gariepinus (Burchell,1822)
Glo. Adv. Res. J. Med. Med. Sci. January 2013 Vol: 2(1): - [Abstract] [Full Text - PDF] (228 KB)
Soheir Mahmouda, Neimat Amera, Nevine Guirguisa, Ahmed H Helmyb, Maisa Shalabya, Wafaa Elkomya and Hoda Sabrya
Potential modulatory role of Glycyrrhiza glabra against hepatic morbidity in chronic murine schistosomiasis
Glo. Adv. Res. J. Med. Med. Sci. February 2017 Vol: 6(1): - [Abstract] [Full Text - PDF] (1,496 KB)
Reviews
Kabeh JD
The Role of Biotechnology in Solving Global Food Crisis
Glo. Adv. Res. J. Med. Med. Sci. October 2012 Vol: 1(3): - [Abstract] [Full Text - PDF] (167 KB)
Marcos Flores-Encarnación, Jennifer Yanine González-Gutiérrez, Daniela Amador-Bravo, Luis Alejandro Bravo-Juárez, and Carlos Cabrera-Maldonado
A bacterial novel quinone participating in multiple functions in higher organisms
Glo. Adv. Res. J. Med. Med. Sci. July 2014 Vol: 3(7): - [Abstract] [Full Text - PDF] (143 KB)
Original Research Articles
Azza A El-Masry, Mokhtar I Yousef, Hussein K Hussein, Nessrin AM Kheirallah and Nico M van Straalen
L-carnitine protects against oxidative stress induced by sublethal exposure to the synthetic pyrethroid, lambda-cyhalothrin, in rats
Glo. Adv. Res. J. Med. Med. Sci. July 2014 Vol: 3(2): - [Abstract] [Full Text - PDF] (281 KB)
Youqin Kong Liqiao Chen, Erchao Li, Zhenyu Du, Zhili Ding.
Growth and antioxidant activity of juvenile oriental river prawn Macrobrachium nipponense, fed diets containing different copper levels under nitrite exposure
Glo. Adv. Res. J. Med. Med. Sci. May 2014 Vol: 3(5): - [Abstract] [Full Text - PDF] (158 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Tugbobo OS on Google Scholar
Tugbobo OS on Pubmed
Abegunde SM on Google Scholar
Abegunde SM on Pubmed
Idowu KS on Google Scholar
Idowu KS on Pubmed

Statistics

Viewed 68
Printed 7
Downloaded 41
Powered By iPortal Works