Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
October 2018, Vol. 7(8), pp. 165-168
Copyright © 2018 Global Advanced Research Journals

 

Full Length Research Article

Protection by Dialium indium (Fruit) against Pb2+-Induced Microcytic Anemia and Chromosome Aberration in Albino Rats In Vivo

Tugbobo O.S.*, Abegunde S.M. and  Idowu K.S.

Department of Science Technology, Federal Polytechnic, Ado-Ekiti, Nigeria

*Corresponding Author E-mail: tugbobooladimeji@yahoo.com 

Accepted 24 October, 2018 

Abstract

The chelating ability and anticlastogenic potential of Dialium indium aqueous extract on lead-induced microcytic anemia and chromosome aberration was investigated in albino rats. Twenty rats were randomly grouped into four where rats in group B were treated daily with oral administration of 2.5mg/kg lead acetate, animals in C were fed simultaneously with 2.5mg/kg lead acetate and 200mg/kg Dialium indium extract while group D animals were fed 200mg/kg Dialium indium extract only and animals in group A served as control. The animals were sacrificed after four weeks of exposure and the toxicological effect of lead and the plant extract on hemoglobin-enzymes and chromosomes were equally assessed via micronucleus assay. The results from hematogical and micronucleus assay suggest that lead could possibly induce microcytic-anemia and as well initiate the development of chromosome aberration in albino rats as observed in group B animals, while the extract offered protective effect to animals in group C. However, the extract anticlastogenic and chelating potential was in synergy with the activity of hemoglobin-enzymes as the frequency of chromosome aberration was significantly (P<0.05) low in group D animals compared to those in group B. 

Keywords: Microcytic anemia, Dialium indium, lead acetate, anticlastogenic, hemoglobin-enzymes.

 

References

Aguawa CN, Mittal GC (1981). Study of antiulcer activity of aqueous extract of leaves of Pyrenacanthia staudtii using various models of experiment gastric ulcer in rats. Eur. J. Pharmacol. 74: 215-220.

Das T, Roychoudhury A, Sharma A, Talukder G (1993). Modification of cytotoxic effect of arsenic by crude extract of garlic in mice. Inter. J. Pharmacognosy, 31: 316-320.

Fejes S, Blazovics A, Lugasi A, Lemberkovics E, Petri G, Kery A (2000). In vitro antioxidant activity of Anthriscus cerefolium (Hoffin) extracts. J. Ethnopharmacol. 69: 259-265.

Humphrey DI (1991). Effect of exposure to excessive quantity of lead on animals. Br. Vet. J, 147: 18-30.

Leonard A (1991). Heavy metals inhibitory effect on thiols-enzymatic groups. Brit. Med. J. 203-208.

Preston RJ, Dean BJ, Gallowxy S, Holden H, Mcfee AF, Shlby M (1987). Mammalian in vivo cytogenetic assay analysis of chromosome aberration in bone marrow cells. Mutat. Res. 189: 157-165.

Rimbach G, Hohler D, Fischer A, Roy S (1999). Methods used to access free radicals and oxidative stress in biological system. Arch. Tiereinahr. 53: 203-222.

Semiz A, Sen A (2007). Antioxidant and chemoprotective properties of bitter melon fruit extract. Afri. J. Biotechnol. 6: 273-277.

Sharma A, Talukder G (1987). Effect of metals on chromosomes of higher organisms. Environ. Mutagen. 9: 191-226.

Sharma AK, Sharma A (1994). Chromosome techniques; manual of Hardwood Publishers, pp.368, Switzerland.

Sofidiya MO, Odukoya OA, Familoni OB, Inya-Agha ST (2006). Free radicals scavenging activity of some Nigerian medicinal plant extracts. Park. J. Biol. Sci. 9: 1438-1441.

Sokal RR, Rohlf FI (1987). Single classification of analysis of variance. Introduction to Biostatistics pp. 363 W.H. Freeman San Francisco.

Stohs ST, Bagchi D (1995). Free radicals, Biol. Med. 18: 321-336.

Tugbobo OS, Oloyede OI, Adewale  OB (2014).Dietary protection by garlic against lead-induced oxidative stress.and genetic birth defects. Oxid. Antioxid. Med. Sci. 3 (1): 79-82


Related Articles


Original Research Articles
Valéria Baccarin Ianiski, Ligia Beatriz Bento Franz, Juliana Schneider, Matias Nunes Frizzo and Eliane Roseli Winkelmann
Oxidative stress and power in patients post - kidney transplantation: a cross study
Glo. Adv. Res. J. Med. Med. Sci. October 2015 Vol: 4(10): - [Abstract] [Full Text - PDF] (157 KB)
Divine A Onobrudu, Jude C Ikewuchi and Eugene N Onyeike
Moderation of Ocular Markers of Oxidative Stress of Selenite Induced Cataractous Pups by Boswellia Dalzielii Hutch Extract
Glo. Adv. Res. J. Med. Med. Sci. December 2016 Vol: 4(2): - [Abstract] [Full Text - PDF] (160 KB)
Wafaa El-komy1, Soheir Mahmoud1, Hoda Sabry1, Soheir Mansy2 and Nevine Guirguis1
Changes in parasitic load, phagocytosis and ultra-structural pattern in experimental cryptosporidiosis following combined (Antox and Nitazode) treatment
Glo. Adv. Res. J. Med. Med. Sci. March 2016 Vol: 5(3): - [Abstract] [Full Text - PDF] (3,544 KB)
Azza A El-Masry, Mokhtar I Yousef, Hussein K Hussein, Nessrin AM Kheirallah and Nico M van Straalen
L-carnitine protects against oxidative stress induced by sublethal exposure to the synthetic pyrethroid, lambda-cyhalothrin, in rats
Glo. Adv. Res. J. Med. Med. Sci. July 2014 Vol: 3(2): - [Abstract] [Full Text - PDF] (281 KB)
Luciana do Amaral Gurgel Galeb, Luciana Nakaghi Ganeco, Ana Carolina Fredianelli, Roberta Wagner, Ana Laura Pinto D’Amico Fam, Daniel Carlos Coatti Rocha, Peter Gaberz Kirschnik and Cláudia Turra Pimpão
Acute intoxication by deltamethrin in jundia: emphasis on clinical, biochemical and haematological effects
Glo. Adv. Res. J. Med. Med. Sci. March 2013 Vol: 2(3): - [Abstract] [Full Text - PDF] (192 KB)
Felemban HM, Mujallid MI and AL-Gehani SA
Effect of organophosphate malathion on tissues of quail (Coturnix coturnix)
Glo. Adv. Res. J. Med. Med. Sci. February 2013 Vol: 2(2): - [Abstract] [Full Text - PDF] (1,235 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Tugbobo OS on Google Scholar
Tugbobo OS on Pubmed
Abegunde SM on Google Scholar
Abegunde SM on Pubmed
Idowu KS on Google Scholar
Idowu KS on Pubmed

Statistics

Viewed 1034
Printed 151
Downloaded 457
Powered By iPortal Works