Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
November 2015 Vol. 4(11), pp. 473-476
Copyright © 2015 Global Advanced Research Journals


Full Length Research Paper

Efficacy of three disinfectant agents against contaminating pathogens isolated from public toilets

Omar B Ahmed* and Bassam H Mashat

Department of Environmental and Health Research, the Custodian of the Two Holly Mosques Institute for Hajj and Umrah Research, Umm Al-Qura University, Makkah Al-Mukaramah, Saudi Arabia.

*Corresponding Author E-mail:; Telephone: 966125572855

Accepted 20 November, 2015


The control of infections in public toilets is a matter of great concern and a major challenge specially places of mass gathering. The study aimed to evaluate the antibacterial activity of three disinfectant agents against contaminating pathogens isolated from public toilets surfaces in Makkah city, Saudi Arabia. Hydrogen peroxide, Polyhexamethylene guanidine (PHMGH) and Sodium hypochloride were tested toward selected bacteria isolated from toilets surfaces using MIC technique. The test organisms were S. aureus, E. faecalis, E. coli and P. aeruginosa. The results showed that MIC values of PHMGH toward isolates were 0.047%, 0.047%, 0.047% and 0.0934% respectively while the MIC values of hydrogen peroxide were 0.0934%, 0.0934%, 0.186% and 0.0934% respectively whereas the MIC values of sodium hypochlorite for all these bacteria was 0.0934%. It was concluded that the PHMGH was the most effective disinfectant agent against tested bacteria and Gram positive bacteria were more susceptible than Gram negative bacteria while P.aeruginosa was the most resistant isolate.

Keywords: Public toilets, Disinfectant, Polyhexamethylene guanidine, Hypochlorite, Hydorogen peroxide.

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