Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
November 2017 Vol. 6(11), pp. 292-295
Copyright © 2017 Global Advanced Research Journals

 

Full Length Research Paper

Suture analysis and arterial traction test in dogs fixed on alcohol and preserved on saline solution aiming surgical practice

Eduardo Scott Fragoso Cerqueira1, Marina Emanoella Seruti Pelógia1, Camila Pinho Balthazar Silveira2, Alisson Senna Fechis1, Thiago André Salvitti de Sá Rocha1, José Luiz Laus2 and Fabrício Singaretti de Oliveira1*

1Department of Animal Morphology and Physiology, Faculty of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, São Paulo, Brazil.
2Department of Veterinary Clinics and Surgery, Faculty of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, São Paulo, Brazil.

*Corresponding Author E-mail: singaretti@fcav.unesp.br, singaretti@ig.com.br; Phone/ Fax: 551632097333

Accepted 10 November, 2017

Abstract

There are several fixative solutions to preserve corpses for surgical use and lots of them uses formalin. The objective of this research was to determine, in dog corpses, the best fixative period in ethylic alcohol (EA) and preservation in sodium chloride aqueous solution 30% (SCAS 30%), aiming the arterial micro-surgical training. Corpses of five groups (G1 to G5) were fixed with EA, and put in boxes containing EA for 30 (G2), 60 (G3), 90 (G4) or 120 days (G5). After that, each group was preserved in SCAS 30% for 120 days. The control group (G1) was composed of corpses without fixation/ preservation. At the end of each period, two fragments of common carotidartery per corpse were collected, for traction test. Immediately after the collection, the femoral arteries were evaluated (by 2 people) regarding the suture quality in binocular surgical microscope, and attributed scores from 0 (bad) to 5 (excelente), regarding the fresh samples. Although, ethylic alcohol has been used as fixative, there were few changes regarding the traction tests in the common carotid arteries. Regarding the conservation in SCAS 30%, there were changes in relation to the control group at 60 and 90 days of conservation in saline solution, but not at 30 and 120 days. As the score at the 30 days group was higher than the 120 days group, we considered G2 (30 days fixation and conservation group) the best group for arterial surgery practice.

Keywords: anatomy, biomechanics, surgery, training.

 

References

Balcombe J (2000). The use ofanimals in highereducation: problems, alternativesandrecommendations. Washington: The Humane Society Press. p.104.

Carpenter LG, Donald L, Piermattei DL, Salman MD, Orton C, Nelson AW, SmeakDD,  Jennings PB, Taylor RA (1991). A comparisonofsurgical training withliveanesthezeddogsandcadavers. Vet. Surg. 20: 373–378.

Goyri-O´neill J, Pais D, Freire De Andrade F, Ribeiro P, Belo A, O’neill A, Ramos S,  Neves Marques C (2013). Improvementoftheembalmingperfusionmethod: The innovationandtheresultsby light andscanningelectronmicroscopy. Acta Med. Port. 26: 188-194.

Groscurth P, Eggli P, Kapfhammer J, Rager GJ, Hornung P, Fasel JDH (2001). Gross Anatomy in the Surgical Curriculum in Switzerland: Improved Cadaver Preservation, Anatomical Models, and Course Development. The Anat. Rec. 265: 254–256.

Guastalli BHL, Nunes TC, Gamón THM, Do Carmo LG, Oliveira FS, Del Quiqui EM (2012). Quantificação do enrijecimento muscular de peito de frango causado por formaldeído. Biotemas. 25: 195-197.

Guastalli BHL, Saddi LGC, Zani FL, Nunes TC, Gamon THM, Oliveira FS (2007). Mensuração da textura de tecido muscular fixado e conservado em solução aquosa de formaldeído por 45 dias – Measurementofthetextureoffixedandconservedmuscle in formaldehydeaqueoussolution for 45 days. XXXIV Congresso Brasileiro De Veterinária - XXXIV VeterinaryBrazilianCongress (Santos, Brazil).

Laflamme DP (1997). Developmentandvalidationof a bodycondition score system for dogs. CaninePractice. 22: 10-15.

Mathews KG, Riley K, Lascelles BDX, Dernell WS (2010). PreparationofCanineandFelineCadavers for SurgicalLaboratories. VetSurg. 39: 224–225.

Nunes TC, Oliveira FS, Gamon THM, Guastalli BHL, Carmo LG, Del Quiqui EM (2011). Análise da textura de músculos peitorais submetidos á fixação e conservação em álcool. Braz. J. Vet. Res. An. Sci. 48: 464-467.

Oliveira FS (2014). Assessing the effectiveness of 30% sodium chloride aqueous solution for the preservation of fixed anatomical specimens: a 5-year follow-up study. J. Anat. 225: 118-121.

Paixão RL (2008). Métodos substitutivos ao uso de animais vivos no ensino - Repensando o que aprendemos com os animais no ensino. CienVet Trop. 11: 88-91.

Rodrigues H (2010). Técnicas Anatômicas. Vitória-ES, Brazil: GM Gráfica & Editora.

Sampaio FJB (1989). Estudo do crescimento do rim humano durante o período fetal – Study of the human kidney growth during fetal life. Thesis (Doctoral). São Paulo State Medicine College, Brazil.

Silva RMG, Matera JM, Ribeiro AACM (2007). New Alternative Methods to Teach Surgical techniques for Veterinary Medicine StudentsdespitetheAbsenceof Living Animals. Isthatan Academic Paradox? Anat. Histol. Embryol. 36: 220–224.

Silva RMG, Matera JM, Ribeiro AACM (2004). Preservation of cadavers for surgical technique training. Vet. Surg. 33: 606–608.

 

Related Articles


Original Research Article
Ismael Quiroz Guerrero and Arturo Pérez Vázquez
Knowledge and Preference of Medicinal Plants in three Rural Communities of Tezonapa, Veracruz, Mexico
Glo. Adv. Res. J. Med. Med. Sci. April 2016 Vol: 5(4): - [Abstract] [Full Text - PDF] (381 KB)
Case Report
Dan Andrei Mitrea, Ana-Maria Vladila, Sanda Maria Nica, Ioan Buraga, Gabriela Mihailescu and Emilia Diaconu
Esthesioneuroblastoma presenting as diplopia and proptosis in a young female patient. Case report and review of the literature
Glo. Adv. Res. J. Med. Med. Sci. December 2014 Vol: 3(12): - [Abstract] [Full Text - PDF] (715 KB)
Original Research Article
Chika O Duru, Ovuirororie E Ederiane and Felix O Akinbami
Child sexual abuse: a review of cases presenting at the out patient clinic of a tertiary health centre in Bayelsa State, Nigeria
Glo. Adv. Res. J. Med. Med. Sci. November 2014 Vol: 3(11): - [Abstract] [Full Text - PDF] (128 KB)
Review
Jean-Claude Garcia-Zamor
A Theoretical Guideline to define Transparency and Accountability in Public Organizations
Glo. Adv. Res. J. Med. Med. Sci. December 2012 Vol: 1(7): - [Abstract] [Full Text - PDF] (199 KB)
Case Report
Awe JAA
Self knotting of Nasogastric tube: an unusual and rare complication.
Glo. Adv. Res. J. Med. Med. Sci. May 2014 Vol: 3(4): - [Abstract] [Full Text - PDF] (145 KB)
Original Research Article
Fernando Mejia Sanchez, Marisol Rodríguez Albarrán, J. Amado López Arriaga and Julieta Castillo Cadena
Heterogeneity of GST enzymatic activity before and after treatment in patients with breast cancer: pilot study
Glo. Adv. Res. J. Med. Med. Sci. July 2017 Vol: 6(7): - [Abstract] [Full Text - PDF] (311 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Eduardo Scott Fragoso Cerqueira on Google Scholar
Eduardo Scott Fragoso Cerqueira on Pubmed
Marina Emanoella Seruti Pelógia on Google Scholar
Marina Emanoella Seruti Pelógia on Pubmed
Camila Pinho Balthazar Silveira on Google Scholar
Camila Pinho Balthazar Silveira on Pubmed
Alisson Senna Fechis on Google Scholar
Alisson Senna Fechis on Pubmed
Thiago André Salvitti de Sá Rocha on Google Scholar
Thiago André Salvitti de Sá Rocha on Pubmed
José Luiz Laus on Google Scholar
José Luiz Laus on Pubmed
Fabrício Singaretti de Oliveira on Google Scholar
Fabrício Singaretti de Oliveira on Pubmed

Statistics

Viewed 94
Printed 21
Downloaded 62
Powered By iPortal Works