Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
December 2016 Special Issue Vol. 5(12), pp. 287-291
Copyright © 2016 Global Advanced Research Journals

Full Length Research Paper 

CTX-M-1 and CTX-M-55 Producing Escherichia coli Isolated from Broiler Feces in Poultry Slaughterhouse, Bogor, West Java Province

Denny Widaya Lukman¹*, Mirnawati Bachrum Sudarwanto¹, Trioso Purnawarman¹, Hadri Latif¹, Herwin Pisestyani¹, Eddy Sukmawinata¹, and Ömer Akineden²

¹Laboratory of Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, West Java, Indonesia
²Dairy Sciences, Institute of Veterinary Food Science, Justus-Liebig University Giessen, Giessen, Germany

*Corresponding Author E-mail: dennylukman@hotmail.com; Phone / Fax: +62 251-625588

Accepted 14 December, 2016

Abstract

This study was aimed to determine the occurrence of CTX-M-1 and CTX-M-55 producing Escherichia coli isolated from broiler feces in Bogor, West Java, Indonesia.  A total of 200 broiler fecal samples were collected from poultry slaughterhouses from March to April 2016. Presence of extended-spectrum beta-lactamase (ESBL) producing E. coli was detected by disc diffusion test based on the recommendation from Clinical and Laboratory Standards Institute. Bacterial strains which were confirmed as producing ESBLs were further analyzed for the presence of bla genes of the ESBL by polymerase chain reaction (PCR). The results showed that CTX-M producing E. coli isolates were detected in 12 samples from 200 samples (6.0%). The β-lactamase genes detected included CTX-M-1 (n = 6) and CTX-M-55 (n = 6).  Most (95.8%) of the CTX-M producing E. coli isolates showed multidrug resistance phenotypes to at least three antibiotics. The highest incidence of antibiotics resistance was against penicillin G (100.0%), followed by gentamicin (79.2%), tetracycline (79.2%), streptomycin (70.8%), enrofloxacin (70.8%), ciprofloxacin (62.5%), trimethoprim-sulfamethoxazole (45.8%), and polymyxin B (16.7%). The occurance of CTX-M ESBL among fecal isolates could be a potential risk factor to public health.

Keywords: broiler feces, CTX-M, Escherichia coli, poultry slaughterhouse