Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
February 2015 Vol. 4(2), pp. 078-082
Copyright © 2015 Global Advanced Research Journals

 

Full Length Research Paper

Sensitivity of aqueous and freeze-dried DAT (AQ-DAT and FD-DAT) in diagnosis of visceral leishmaniasis in Sudan- Possibility of adapting the DAT (aqueous antigen) as a rapid test by using blood spot on filter paper

Mohammed H F Shalayel1*, Amin A A Elbadawi2, Ietimad AM Ayed3, Khalid O Abosalif4, Gadallah Modawe3, Ihab Hamed Nourein5, Nuha G Ahmed6 and Sayda H El Safi7

1Professor of Biochemistry, College of Medicine, Najran University, KSA.
2Department of Biochemistry, College of Medicine, Najran University, KSA.
3Faculty of Medicine and Health Sciences, Omdurman Islamic University, Sudan.
4Omdurman Islamic University, Sudan
5College of Applied Medical Sciences, Najran University, KSA.
6Department of Biochemistry, Faculty of Medicine, University of Khartoum, Sudan.
7Department of Microbiology, Faculty of Medicine, University of Khartoum, Sudan.

*Corresponding Author E-mail: drmhfs@hotmail.com

Accepted 09 February, 2015

Abstract

Leishmaniasis is the second largest parasitic killer in the world. In developing countries with large numbers of patients in rural areas, simple diagnostic tools are necessary for field use. Our study aimed to compare the performance of the aqueous with freeze-dried DAT (AQ-DAT and FD-DAT) in diagnosis of visceral leishmaniasis and to investigate the possibility of adapting the DAT (aqueous antigen) as a rapid test by using different sampling method (blood spot on filter paper and serum from venous blood) with different elution times and different incubation times, and to determine the significant titre in each method. The sensitivity of FD-DAT and AQ-DAT at a cut-off titre 1:3200 was found to be 100% as both antigens gave a significant diagnostic titre for all of the parasitologically confirmed VL cases. We concluded that AQ-DAT and FD-DAT have a very high sensitivity for the diagnosis of visceral leishmaniasis at a cutoff titre of 1:3200, with a fair agreement between the two tests (Kappa measure of agreement =0.21). We also noticed that using filter papers have excellent results especially when taking an elution time of eighteen hours.

Key words: Visceral leishmaniasis (VL), aqueous DAT (AQ-DAT), freeze-dried (FD-DAT).

 

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