Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
April 2015 Vol. 4(4), pp. 176-182
Copyright © 2015 Global Advanced Research Journals
Full Length Research Paper
Detection of Helicobacter pylori DNA in purified water for drinking
Alejandro Ulises Herrera-Romero1, Germán Rubén Aguilar-Gutiérrez2 and Marcos Flores-Encarnación1*
1Laboratorio de Microbiología Molecular y Celular. Edif. 323G. Biomedicina, Facultad de Medicina, Benemérita Universidad Autónoma de Puebla. Puebla, Puebla. México.
2Centro de Investigación sobre Enfermedades Infecciosas. Instituto Nacional de Salud Pública. Cuernavaca, Morelos. México.
*Corresponding Author E-mail: mflores31@hotmail.com, Phone: +52222 5530754
Accepted 01 April, 2015
Abstract
H. pylori is a Gram negative bacterium microaerophilic that is associated to digestive human diseases such as gastritis, peptic ulcer, mucosa-associated lymphoid tissue lymphoma; this bacterium is considered a risk factor in the development of gastric cancer. Different vias have been proposed by transmission of H. pylori. H. pylori has been detected in well water, municipal water, treated wastewater and drinking water in developing countries. The present study shows an evidence of the occurrence of H. pylori through detection of DNA of this bacterium in purified water for drinking of Puebla city. In this work we apply nested PCR to the detection of H. pylori using primers that specifically amplify the gene 16S rRNA.
Keywords: Helicobacter pylori, water, 16S rRNA, detection, drinking, PCR, nested.
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