Global Advanced Research Journal of Medicine and Medical Science (ISSN: 2315-5159)
April 2015  Vol. 4(4) pp. 207-212
Copyright © 2015 Global Advanced Research Journals

 

Full Length Research Paper

Molecular Epidemiology of Shigella sonnei Isolated from Clinical cases in Tehran using RAPD-PCR Method

Arman Rostamzad

Department of Biology, Faculty of Science, University of Ilam, Iran.

E-mail: Arostamzad381@yahoo.com; Tel: 0098(84) 32227022; Fax: 0098(84) 32225767

Accepted 23 March, 2015

Abstract

To characterize Shigella clinical strains, especially Shigella sonnei, we studied 80Shigella strains recovered from 495 stool samples of patients with bloody diarrhea in three hospitals in Tehran, Iran, over the period of 2 years from May2010 to June2012. Serological assay classified the Shigella isolates as follows: 60(75%) Shigella sonnei isolates, 15 (18.75%) Shigella flexneri isolates,2 (2.5%) Shigella boydii isolates, and 3 (3.75%) Shigella dysenteriae isolates. In an antibiogram test, all Shigella strains were susceptible to ciprofloxacin, and ceftriaxone. They showed high degrees of sensitivity to nalidixic acid, gentamicin and amikacin. Approximately 98.66% of the Shigella isolates were resistant to co-trimoxazole. The RAPD-PCR fingerprinting was carried out using ARB11 primer. The PCR product was run and visualized in 1% agarose gels and stained with ethidium bromide. The RAPD-PCR profiles were grouped depending on the patterns of the amplified bands. In conclusion, these data mandate local monitoring of drug resistance and its consideration in the empirical therapy of Shigella infections. These results also demonstrated that RAPD-PCR analysis is more reliable than antibiotic susceptibility pattern analysis and could be used as a routine laboratory method for the identification of Shigella species, those causes  epidemic strains in Iran.

Keywords: Shigella, Serotyping, RAPD-PCR.

 

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