Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
April 2016 Issue Vol. 5(4), pp. 103-108
Copyright © 2016 Global Advanced Research Journals

 

Full Length Research Paper

Susceptibility of Lys656Asn Polymorphism of Leptin Receptor Gene to Hypertension in Obese Javanese subjects of Indonesia

Pramudji Hastuti*, Izza Zukhrufia, Made Harumi Padmaswari, Afifah Nuraini and Ahmad Hamim Sadewa

Department of Biochemistry, Faculty of Medicine, Gadjah Mada University, Yogyakarta,  Indonesia.

*Corresponding Author E-mail: hpramudji@yahoo.com, pramudji.has@ugm.ac.id; Tel: +62-81578706213

Accepted 15 April, 2016

Abstract

Hypertension is a multifactorial disease influenced by environmental and genetic factors. Most obese subjects exhibit leptin resistance and increase in adiposity. Leptin has the capacity to increase sympathetic nerve activity (SNA) and further increase blood pressure by binding to leptin receptors. There are polymorphisms of the leptin receptor gene. Lys656Asn polymorphism of leptin receptor (LEPR) gene results from basic amino acid (Lys) to amide amino acid (Asn) substitution. It influences the occurrence of obesity and hypertension. One hundred and six (106) healthy subjects were recruited for this study and were divided into two groups: obese and control groups comprising of 53 subjects each. Non-fasting blood samples were taken to determine the leptin level by the Elisa method and Lys565Asn polymorphism of LEPR gene was determined by PCR-RFLP method. The t-test was then performed to determine the differences in the phenotypic and leptin levels in all groups. This study showed an increase of leptin level significantly in obese group than control. The obese subjects with high blood pressure have shown higher leptin level than control. Lys656Asn genotype subjects showed more elevated blood pressure than Lys656Lys genotype in the obese group. Lys656Asn genotype subjects  in obese group were greater in waist circumference, % body fat and leptin level than in control subjects. In conclusion, Lys656Asn polymorphism of leptin receptor gene is a risk factor for the increase of leptin level and hypertension. This study can be continued by determining some genetic factors related to hypertension and obesity in some ethnic of Indonesia.

Keywords: Susceptibility; Hypertension; LEPR gene; obesity; polymorphism

 

Related Articles


Original Research Articles
Enwuso A Igwe
Shredded Tire Chips Influence on the Rheological Properties of Grade 60 and 70 Bitumen used in Flexible Road Pavements
Glo. Adv. Res. J. Med. Med. Sci. September 2015 Vol: 4(5): - [Abstract] [Full Text - PDF] (172 KB)
Mohsen M Maher, Tarek M Yosef, Wesam A Ibrahim, Maha M Hussein, Manal Mohsen, Ahmed S Khayal, Walaa Hashem, Manal Ghozlan and Emad Abd-Eldayem
Significance of von Willebrand Factor in Compensated and Decompensated Cirrhosis
Glo. Adv. Res. J. Med. Med. Sci. November 2017 Vol: 6(11): - [Abstract] [Full Text - PDF] (269 KB)
Amauri Dalla-Corte, José L Vieira, Irenio G Silva Filho, César L Reichert and Roberta R Dalla-Corte
Factors associated with lower quality of life in Brazilian elderly people
Glo. Adv. Res. J. Med. Med. Sci. January 2015 Vol: 4(1): - [Abstract] [Full Text - PDF] (177 KB)
Maria L Salvetat, Marco Zeppieri, Flavia Miani and Paolo Brusini
Endothelial cell loss and graft survival after Descemet stripping automated endothelial keratoplasty (DSAEK): 6-year follow-up
Glo. Adv. Res. J. Med. Med. Sci. June 2015 Vol: 4(6): - [Abstract] [Full Text - PDF] (127 KB)
Adel S Al-Zubairi, Mohamed A Al-Mamary and Eftekhar Al-Ghasani
The Antibacterial, Antifungal, and Antioxidant Activities of Essential Oil from Different Aromatic Plants
Glo. Adv. Res. J. Med. Med. Sci. September 2017 Vol: 6(9): - [Abstract] [Full Text - PDF] (168 KB)
Review
Gyimah Daniel Agyei, Liu Liping, Jun Wang, Jie Gao
Occurrence Fusarium species, mycotoxins on cereal crops and their health implication
Glo. Adv. Res. J. Med. Med. Sci. October 2018 Vol: 7(10): - [Abstract] [Full Text - PDF] (148 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Pramudji Hastuti on Google Scholar
Pramudji Hastuti on Pubmed
Izza Zukhrufia on Google Scholar
Izza Zukhrufia on Pubmed
Made Harumi Padmaswari on Google Scholar
Made Harumi Padmaswari on Pubmed
Afifah Nuraini on Google Scholar
Afifah Nuraini on Pubmed
Ahmad Hamim Sadewa on Google Scholar
Ahmad Hamim Sadewa on Pubmed

Statistics

Viewed 1735
Printed 582
Downloaded 717
Powered By iPortal Works