Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
April 2016 Issue Vol. 5(4), pp. 103-108
Copyright © 2016 Global Advanced Research Journals


Full Length Research Paper

Susceptibility of Lys656Asn Polymorphism of Leptin Receptor Gene to Hypertension in Obese Javanese subjects of Indonesia

Pramudji Hastuti*, Izza Zukhrufia, Made Harumi Padmaswari, Afifah Nuraini and Ahmad Hamim Sadewa

Department of Biochemistry, Faculty of Medicine, Gadjah Mada University, Yogyakarta,  Indonesia.

*Corresponding Author E-mail:,; Tel: +62-81578706213

Accepted 15 April, 2016


Hypertension is a multifactorial disease influenced by environmental and genetic factors. Most obese subjects exhibit leptin resistance and increase in adiposity. Leptin has the capacity to increase sympathetic nerve activity (SNA) and further increase blood pressure by binding to leptin receptors. There are polymorphisms of the leptin receptor gene. Lys656Asn polymorphism of leptin receptor (LEPR) gene results from basic amino acid (Lys) to amide amino acid (Asn) substitution. It influences the occurrence of obesity and hypertension. One hundred and six (106) healthy subjects were recruited for this study and were divided into two groups: obese and control groups comprising of 53 subjects each. Non-fasting blood samples were taken to determine the leptin level by the Elisa method and Lys565Asn polymorphism of LEPR gene was determined by PCR-RFLP method. The t-test was then performed to determine the differences in the phenotypic and leptin levels in all groups. This study showed an increase of leptin level significantly in obese group than control. The obese subjects with high blood pressure have shown higher leptin level than control. Lys656Asn genotype subjects showed more elevated blood pressure than Lys656Lys genotype in the obese group. Lys656Asn genotype subjects  in obese group were greater in waist circumference, % body fat and leptin level than in control subjects. In conclusion, Lys656Asn polymorphism of leptin receptor gene is a risk factor for the increase of leptin level and hypertension. This study can be continued by determining some genetic factors related to hypertension and obesity in some ethnic of Indonesia.

Keywords: Susceptibility; Hypertension; LEPR gene; obesity; polymorphism


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