Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
April 2017 Vol. 6(4), pp. 071-074
Copyright © 2017 Global Advanced Research Journals

 

Full Length Research Paper  

Polymerase chain reaction and Quanti FERON enhance acid-fast bacillus stain-based rapid detection of Mycobacterium tuberculosis isolated from displaced Iraqi patients

Mohemid M Al-Jebouri* and Burhan M Ali

Department of Microbiology, College of Medicine, University of Tikrit, Tikrit, Iraq.

*Corresponding Author E-mail: profaljebouri@yahoo.com

Accepted 12 April, 2017

Abstract

For exactly  detection  of  the  TB  cases  recently  a novel  polymerase  chain  reaction  ( PCR)  based   diagnostic  kit  has  been  developed. It is based  on the  nucleic  acid  amplification (NAA) of  specific region  of  Mycobacterium DNA. Quanti FERON-TB test, (QFT)  an in vitro diagnostic test that measures  a constituents of cell-mediated  immune  reactivity to M. tuberculosis was  approved  by  food  and  Drug  administration (FAD) as  an aid for identifying Mycobacterium tuberculosis infection. In the current study there were 50 patients (18 displaced and 32 non displaced TB patients) and 40 healthy control. The patient were examined for the presence of TB utilizing Quanti FERON-TB Gold In-Tube assay, polymerase chain reaction (PCR), AFB smear and T.B. culture. It was found that the frequency of positivity of acid-fast stain, culture  and Quanti FERON for displaced and non-displaced patients  was 36 , 33.3 and 100 and 64 , 66.7 and 100 % respectively. The positivity towards polymerase chain reaction for primers  IS6110 and MPB64 for displaced patients was 37.5 and 100% respectively whereas for non displaced patients was 14.3 and 100 % respectively too. The PCR test for the presence of primer MPB64  and Quanti FERON test were 100% positive for all mycobacterial isolates tested from displaced and non displaced patients whereas other identification tests revealed variations in reproducibility.

Keywords: Acid-fast bacilli detection, PCR, Quanti FERON, Culture, Iraqi refugees.

 

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