Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
April 2017 Vol. 6(4), pp. 071-074
Copyright © 2017 Global Advanced Research Journals
Full Length Research Paper
Polymerase chain reaction and Quanti FERON enhance acid-fast bacillus stain-based rapid detection of Mycobacterium tuberculosis isolated from displaced Iraqi patients
Mohemid M Al-Jebouri* and Burhan M Ali
Department of Microbiology, College of Medicine, University of Tikrit, Tikrit, Iraq.
*Corresponding Author E-mail: email@example.com
Accepted 12 April, 2017
For exactly detection of the TB cases recently a novel polymerase chain reaction ( PCR) based diagnostic kit has been developed. It is based on the nucleic acid amplification (NAA) of specific region of Mycobacterium DNA. Quanti FERON-TB test, (QFT) an in vitro diagnostic test that measures a constituents of cell-mediated immune reactivity to M. tuberculosis was approved by food and Drug administration (FAD) as an aid for identifying Mycobacterium tuberculosis infection. In the current study there were 50 patients (18 displaced and 32 non displaced TB patients) and 40 healthy control. The patient were examined for the presence of TB utilizing Quanti FERON-TB Gold In-Tube assay, polymerase chain reaction (PCR), AFB smear and T.B. culture. It was found that the frequency of positivity of acid-fast stain, culture and Quanti FERON for displaced and non-displaced patients was 36 , 33.3 and 100 and 64 , 66.7 and 100 % respectively. The positivity towards polymerase chain reaction for primers IS6110 and MPB64 for displaced patients was 37.5 and 100% respectively whereas for non displaced patients was 14.3 and 100 % respectively too. The PCR test for the presence of primer MPB64 and Quanti FERON test were 100% positive for all mycobacterial isolates tested from displaced and non displaced patients whereas other identification tests revealed variations in reproducibility.
Keywords: Acid-fast bacilli detection, PCR, Quanti FERON, Culture, Iraqi refugees.
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