Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
April 2018, Vol. 7(4), pp. 077-084
Copyright © 2018 Global Advanced Research Journals

 

Full Length Research Article

In vitro cytotoxicity of lignans isolated from Ficus citrifolia P.Miller (Moraceae) on C6 glioma cell line

 

Ana Cristina Baetas1, Luís Fábio dos Santos Gomes 2, Ana Paula Drummond Rodrigues3,6, Luís Antônio Loureiro Maués2, Maria Elena Crespo-Lopez5,9, Barbarella de Matos Macchi2,9, Edilene Oliveira Silva3,7, Mara Sílvia Pinheiro Arruda4 and José Luiz Martins Do Nascimento2,3,8,9*

1Laboratório de Insumos Farmacêuticos da Amazônia, Instituto de Ciências da Saúde, Universidade Federal do Pará, Belém, Pará, Brasil.
2Laboratório de Neuroquímica Molecular e Celular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brasil.
3Laboratório de Biologia Estrutural/Laboratório de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brasil.
4Laboratório de Química-Pesquisa, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Belém, Pará, Brasil.
5Laboratório de Farmacologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brasil.
6Instituto Evandro Chagas, Laboratório de Microscopia Eletrônica, SVS, MS, Av. Almirante Barroso 492, Bairro Marco, 66090-000 Belém, Pará, Brazil
7Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagem, Ilha do Fundão, 21941 902 Rio de Janeiro, Rio de Janeiro, Brazil
8Grupo de pesquisa em Neurociências, Universidade CEUMA, São Luís, Brasil
9Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação (INCT – NIM), Rio de Janeiro, Rio de Janeiro, Brasil

*Corresponding Author E-mail: jlmn@ufpa.br; Cell Phone: 55(91) 981623989, Fax: 55(91) 3201 7601

Accepted 25 April, 2018 

Abstract

Matairesinol and arctigenin, two dibenzylbutyrolactonelignans, were isolated by chromatographic analysis of the CH2Cl2 extract from the leaves of Ficus citrifolia. Structural identification was performed using typical spectroscopic methods, NMR 1H and NMR 13C, in addition to DEPT, NOE-diff, HETCOR, COSY 1H x 1H and HMBC, confirmed by literature comparison. Furthermore, to date, no reports have described the phytochemical investigation of Ficus citrifolia. Previous studies have suggested that dibenzylbutyrolactonelignans exert a preventive effect against different type of cancers such as breast, prostate and colon.  In this study, we analyzed the cytotoxic effects of matairesinol and arctigenin and the induction of apoptosis of C6 rat glioma cells. These compounds produced a dose-dependent reduction in the viability of C6 cells, as determined by an MTT assay and were not cytotoxic against astrocyte cell cultures from rat cerebral tissue. For ultrastructural analysis, C6 cells were incubated with 50 µM and 250 µM of arctigenin and matairesinol. Treated cells presented altered morphology, condensation of nuclear chromatin into irregular clumps, swollen mitochondria, and disorganized internal-membrane cristae. However, the nuclear and plasma membranes remained intact. These morphological changes are characteristic of apoptotic cellular death. In addition, these treatments did not significantly affect astrocyte viability. Thus, these results suggest that both dibenzylbutyrolactonelignans have significant anti-proliferative and apoptotic effects on glioma cells and may contribute as selective agents against brain cancer.   

Keywords: Ficus citrifolia; Moraceae; dibenzylbutyrolactonelignan; matairesinol; arctigenin; cytotoxicity, glioma cells (C6).

 

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