Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
May 2016 Issue Vol. 5(5), pp. 143-149
Copyright © 2016 Global Advanced Research Journals

 

Full Length Research Paper

Probiotic Potency and Molecular Identification of Lactic Acid Bacteria Isolated from Bali Cattle’s Colon, Indonesia

I Wayan Suardana1*, Annisa Putri Cahyani2 and Komang Januartha Putra Pinatih3

1Department of Veterinary Public Health, Faculty of Veterinary Medicine, Udayana University, Denpasar. Jl. PB. Sudirman Denpasar-Bali. 80232 Indonesia.
2Undergraduate Student of Veterinary Public Health, Faculty of Veterinary Medicine, Udayana University, Denpasar. Jl. PB. Sudirman Denpasar-Bali. 80232 Indonesia.
3Department of Microbiology, Faculty of Medicine, Udayana University Denpasar. Jl. PB. Sudirman  Denpasar-Bali. 80232 Indonesia.

*Corresponding Author E-mail: iwayansuardana22@yahoo.com, wayan_suardana@unud.ac.id; Mobile: +62 81338621615; Tlp/Fax : +62(361) 223791

Accepted 19 May, 2016

Abstract

Lactic acid bacteria (LAB) is a group of bacteria which able to produce lactic acid as a main product. LAB  normally live  in the digestive tract of humans and animals, and generally use as probiotics. On the other hand, Bali cattle are known has highly adaption to poor quality of feed so that it is suggested that specific types of LAB can be found. The aims of this study was to determine the probiotics producing by  LAB isolates 18A isolated from colon of Bali cattle as well as to identify of isolate by analysis of the 16S rRNA gene sequence. Probiotic potency analysis was performed by cultivation of LAB isolates 18A on the Man Rogosa Sharpe Broth (MRSB) medium, continued by Gram staining and catalase test. Antimicrobial activities  of isolate were done against pathogenic bacteria (E. coli KL 48 (2) and S. aureus). Furthermore, genetic analysis was initiated by DNA isolation, amplification of the 16S rRNA gene, and sequencing. The result showed that LAB isolate 18A has inhibitory efficacy against gastrointestinal pathogens such as Escherichia coli KL 48 (2) and Staphylococcus aureus by 18,8% and 28,06% respectively. Moreover, genetic analysis showed the LAB isolate 18A as Enterococcus durans with the similarity level of 99%, and a value of 100 bootstrapping.

Keywords: Lactic acid bacteria, 16S rRNA gene, antimicrobial activity, Bali cattle’colon.

 

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