Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
June 2017 Vol. 6(6), pp. 103-110
Copyright © 2017 Global Advanced Research Journals


Full Length Research Paper 

Effects of Clove Oil on Liver and Antioxidant Status of Streptozotocin-Induced Diabetic Rats

Hana M. Gashlan* and Asma B. Al-Beladi

Department of Biochemistry, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.

*Corresponding Author E-mail:,

Accepted 19 June, 2017


This study was design To evaluate the hepatoprotective and antioxidant effect of low and high doses of clove oil on diabetic rats. Forty female albino rats were equally divided into four groups. The first group served as control (G1). The other 3 groups were intraperitoneal injected with 45 mg/kg body weight of streptozotocin (STZ) to induce diabetes. One diabetic group (G2) kept untreated and served as positive control. The other diabetic groups (G3 and G4) were treated with 300 mg/kg and 600 mg/kg of clove oil respectively. Results revealed that the STZ-induced diabetic group exhibited high significant increase in the activities of the liver enzymes (alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase) as well as  reduction in the activities of catalase, superoxide dismutase and glutathione reductase compared to the control group. Diabetic groups treated with different doses of clove oil showed a decrease in the activities of liver enzymes in and increase in antioxidant enzymes activities compared to untreated diabetic group. Histopathological examination of liver tissues of diabetic rats indicated sever changes. However, their changes were overcome by clove oil treatment and the majority of the cells tend to be normal. Clove oil supplementation may act as antioxidant agents and could be an excellent adjuvant support in therapy of diabetes mellitus and its complications.

Keywords: Diabetes, albino rats, clove oil, streptozotocin, diabetes mellitus, liver enzymes, antioxidant and histopathology.



Abozid M, EL-Sayed S (2013). Antioxidant and protective effect of clove extracts and clove essential oil on hydrogen peroxide treated rats", Int. J. Chem. Tech. Res. 5(4): 1477-1485.

Adefegha S, Oboh G, Adefegha O, Boligon A, Athayde M (2014). Antihyperglycemic, hypolipidemic, hepatoprotective and antioxidative effects of dietary clove (Szyzgium aromaticum) bud powder in a high-fat diet/streptozotocin-induced diabetes rat model", J. Sci. Food and Agric. 94 (13): 2726-2737.

Agbaje E, Adeneye A, Daramola A (2010). Biochemical and toxicological studies of aqueous extract of Syzigium aromaticum (L.) Merr. and Perry (Myrtaceae) in rodents", Afr. J. Trad. Complementary and Alternative Med. 6(3).

Al-Attar A (2010). Physiological effects of some plant oils supplementation on streptozotocin-induced diabetic rats". Res. J. Med. Med. Sci. 5 (1): 55-71.

Al-Attar A, Zari T (2007). Modulatory Effects of Ginger and Clove Oils on Physiological Responses in Streptozotocin-Induced Diabetic Rats". Int. J. Pharmacol. 3(1): 34-40.

Alimohammadi S, Hobbenaghi R, Javanbakht J, Kheradmand D, Mortezaee R, Tavakoli M, Khadivar F, Akbari H (2013). Protective and antidiabetic effects of extract from Nigella sativa on blood glucose concentrations against streptozotocin (STZ)-induced diabetic in rats: an experimental study with histopathological evaluation". Diagnostic Pathol. 8(1): 137.

Alma M, Erta M, Nitz S, Kollmannsberger H (2007). Chemical composition and content of essential oil from the bud of cultivated turkish clove", Bio. Resources. 2(2): 265-269.

Aydin M, Celík S (2012). Effects of lycopene on plasma glucose, insulin levels, oxidative stress, and body weights of strep­tozotocin-induced diabetic rats. Turk. J. Med. Sci.

Bancroft D, Cook C (1998). Manual of histotechnological techniques", Edited by: Churchill Livingstone., New York : 243.

Basha R, Sankaranarayanan C (2015). Protective role of caryophyllene, a sesquiterpene lactone on plasma and tissue glycoprotein components in streptozotocin-induced hyperglycemic rats. Journal of Acute Medicine. 5(1):9-14.

Bolkent S, Sacan O, Karatug A, Yanardag R (2008). The effects of vitamin B6 on the liver of diabetic rats: A morphological and biochemical study", IUFS J Biol.  67(1):1-7.

Buege J, Aust S (1978). Microsomal lipid peroxidation. Methods in enzymology. 52:302-310.‏

Chaudhry Z, Chaudhry S, Naseer A, Chaudhry F (2013). Effect of Syzygium aromaticum (clove) extract on blood glucose level in streptozotocin induced diabetic rats. Pakistan Armed Forces Med. J. 63(3):323-328.

Cortés-Rojas D, de Souza C, Oliveira W (2014). Clove (Syzygium aromaticum): a precious spice", Asian Pacific J. Tropical Biomed. 4(2): 90-96.

Daniel E, Mohammed A, Tanko Y, Ahmed A, Adams M, Atsukwei D (2015). Effects of lycopene on thyroid profile in streptozotocin-induced diabetic Wistar rats. Eur. J. Biotechnol. Biosci. 3(1):21-28.

Erden M, Bor N (1984). Changes of reduced glutathion, glutathion reductase, and glutathione peroxidase after radiation in guinea pigs". Biochem. Med. 31(2): 217-227.

Fayed D (2013). Improvement of the efficiency of praziquantel by Citharexylum quadrangular extract and micronutrients in murine schistosomiasis", PH.D, Ain Shams University. Egypt.

 Gayathri M, Kannabiran K (2008). Antidiabetic and ameliorative potential of Ficus bengalensis bark extract in streptozotocin induced diabetic rats", Ind. J. Clin. Biochem. 23(4): 394-400.

Guven A, Yavuz O, Cam M, Ercan F, Bukan N, Comunoglu C, Gokce F (2006). Effects of melatonin on streptozotocin-induced diabetic liver injury in rats", Acta Histochemica. 108(2): 85-93.

Hadi Salih D (2013). Study of Liver Function Tests and Renal Function Tests in diabetic type II patients", IOSR. J. Appl. Chem. 3(3): 42-44.

Hassanen N (2010). Protective effct of cinnamon, clove and ginger spicces or their essential oils on oxidative stress of streptozotocin-induced diavetic rats", Arab Universities J. Agric. Sci. 18, (1):137-154.

John H (1992). Adaptation in natural and artificial systems: an introductory analysis with applications to biology, control, and artificial intelligence", John Henry Holland :MIT press.

Kadarian C, Broussalis A, Miño J, Lopez P, Gorzalczany S, Ferraro G, Acevedo C (2002).  Hepatoprotective activity of Achyrocline satureioides (Lam) D. C.", Pharmacol. Res. 45(1): 57-61.

Khattab H, Al-Amoudi N, Al-Faleh A (2013). Effect of ginger, curcumin and their mixture on blood glucose and lipids in diabetic rats", Life Sci.  J. 10(4): 428-442. 

Kota N, Panpatil V, Kaleb R, Varanasi B, Polasa K (2012). Dose-dependent effect in the inhibition of oxidative stress and anticlastogenic potential of ginger in STZ induced diabetic rats", Food Chem. 135(4): 2954-2959.

Lubinsky S, Bewley C (1979). Genetics of catalase in drosophila melanogaster: rates of synthesis and degradation of the enzyme in flies aneuploid and euploid for the structural gene", Genetics, 91(4): 723-742.

Mansour H, Newairy A, Yousef M, Sheweita S (2002). Biochemical study on the effects of some Egyptian herbs in alloxan-induced diabetic rats", Toxicol. 170(3): 221-228.

Maritim A, Sanders R, Watkins J (2003). Diabetes, oxidative stress, and antioxidants: A review", J. Biochem. Molecular Toxicol. 17(1): 24-38.

Narasimhulu G, Mohamed J, Reddy K (2014). Antihyperglycemic effect of pimpinella tirupatiensis leaves in streptozotocin-induced diabetic rats", Bulletin of Environ. Pharmacol. Life Sci. 3(2): 5-13.

Ozsoy-Sacan O, Yanardag R, Orak H, Ozgey Y, Yarat A, Tunali T (2006). Effects of parsley (Petroselinum crispum) extract versus glibornuride on the liver of streptozotocin-induced diabetic rats", J. Ethnopharmacol. 104(1-2): 175-181.

Posuwan J, Prangthip P, Leardkamolkarn V, Yamborisut U, Surasiang R, Charoensiri R, Kongkachuichai R (2013). Long-term supplementation of high pigmented rice bran oil (Oryza sativa L.) on amelioration of oxidative stress and histological changes in streptozotocin-induced diabetic rats fed a high fat diet; Riceberry bran oil", Food Chem. 138 (1): 501-508.

Raghavan B, Kumari S (2006). Effect of Terminalia arjuna stem bark on antioxidant status in liver and kidney of alloxan diabetic rats", Ind. J. Physiol. Pharmacol. 50(2): 133-142.

Ramadan M, Asker M, Tadros M (2013). Lipid profile, antiradical power and antimicrobial properties of Syzygium aromaticum oil", Grasas y Aceites. 64(5): 509-520.

Ruiz-Larrea MB, Leal AM, Liza M, Lacort M, De Groot  H (1994). Antioxidant effects of estradiol and 2-hydroxyestradiol on iron-induced lipid peroxidation of rat liver microsomes. Steroids. 59(6):383-388.

Saad E, El-Shahat A, Ahmed G (2015). Clove or green tea administration antagonizes Khat hepatotoxicity in rats" Int. J. Pharm. Pharm. Sci. 7(6): 975-1491.

Schmatz R, Perreira L, Stefanello N, Mazzanti C, Spanevello R, Gutierres J, Bagatini M, Martins C, Abdalla F, Daci da Silva Serres J, Zanini D, Vieira J, Cardoso A, Schetinger M, Morsch V (2012). Effects of resveratrol on biomarkers of oxidative stress and on the activity of delta aminolevulinic acid dehydratase in liver and kidney of streptozotocin-induced diabetic rats", Biochim. 94(2): 374-383.

Shanmugam K, Mallikarjuna K, Nishanth K, Kuo C, Reddy K (2011). Protective effect of dietary ginger on antioxidant enzymes and oxidative damage in experimental diabetic rat tissues", Food Chem. 124(4): 1436-1442.

Sheweita S, Mashaly S, Newairy A, Abdou H, Eweda S (2016). Changes in Oxidative Stress and Antioxidant Enzyme Activities in Streptozotocin-Induced Diabetes Mellitus in Rats: Role ofAlhagi maurorumExtracts", Oxidative Medicine and Cellular Longevity. pp. 1-8.

Shukri R, Mohamed S, Mustapha N (2010). Cloves protect the heart, liver and lens of diabetic rats", Food Chem. 122(4): 1116-1121.

Srinivasan S, Sathish G, Jayanthi M, Muthukumaran J, Muruganathan U, Ramachandran V (2013). Ameliorating effect of eugenol on hyperglycemia by attenuating the key enzymes of glucose metabolism in streptozotocin-induced diabetic rats", Molecular and Cellular Biochem. 385(1-2): 159-168.

Vijayakumar M, Govindarajan R, Rao G, Rao C, Shirwaikar A, Mehrotra S, Pushpangadan P (2006). Action of Hygrophila auriculata against streptozotocin-induced oxidative stress", J. Ethnopharmacol. 104(3): 356-361.

Yilmaz H, Uz E, Yucel N, Altuntas I, Ozcelik N (2004). Protective effect of caffeic acid phenethyl ester (CAPE) on lipid peroxidation and antioxidant enzymes in diabetic rat liver", J. Biochem. Molecular Toxicol. 18(4): 234-238.

Yogalakshmi BP, Viswanathan, Anuradha C (2010). Investigation of antioxidant, anti-inflammatory and DNA-protective properties of eugenol in thioacetamide-induced liver injury in rats", Toxicol. 268(3): 204-212.


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