Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
July 2015 Vol. 4(7), pp. 308-313
Copyright © 2015 Global Advanced Research Journals


Full Length Research Paper

In-vitro osmotic fragility assessment of Trigonella foenum graecum linn. seed extract on sheep erythrocytes

Karuna Priyachitra1, Kantipudi Teja Sri2*, Cheeli Ramesh2, Srinath Nissankararao2 and Ramadevi Bhimavarapu2

1Ph.D Research Scholar,KrshnaUniversity,Machiliatnam
2Sri Siddhartha Pharmacy College, Nuzvid, Krishna district, Andhra Pradesh

*Corresponding Author E-mail:

Accepted 16 June, 2015


The search for new hypoglycaemic compounds for the treatment of diabetes or any condition that leads to hyperglycemia. Several evidences suggest that oxidative stress plays a major role in pathogenesis of disease. Damage to RBC membrane lipid and protein is caused by high levels of free radicals which will finally result in hemolysis. An extract from seeds of Trigonella foenum graecum was evaluated for its protective action against H2O2 induced oxidation and different concentration of NaCl induced hemolysis in normal sheep erythrocytes on treatment with fenugreek extract the oxidative modification in both were found to reduce significantly. The total phenolic content in extract was determined on spectrophotometrically using folin-ciocalfeau procedure and the fenugreek inhibition of lipid peroxidation was concentration dependant upto 160µg/l of extract which contained 1.2mM gallic acid equivalent(GAE) of phenolic compounds these findings demonstrated the sound knowledge regarding patient anti-oxidant property of fenugreek seeds.

Keywords: fenugreek seed extract, osmotic fragility test, sheep red blood cells, lipid peroxidation, hemolysis


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