Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
July 2017 Vol. 6(7), pp. 156-161
Copyright © 2017 Global Advanced Research Journals

 

Full Length Research Paper 

Heterogeneity of GST enzymatic activity before and after treatment in patients with breast cancer: pilot study

Fernando Mejia Sanchez1, Marisol Rodríguez Albarrán2, J. Amado López Arriaga1 and Julieta Castillo Cadena1*

1Centro de Investigación en Ciencias Médicas, Universidad Autónoma del Estado de México, Jesús Carranza No. 205, Col. Universidad, Toluca de Lerdo, México. C.P. 50130.
2Facultad de Química, Universidad Autónoma del Estado de México. Paseo Colón esq Paseo Tollocan S/N. Toluca de Lerdo, México. C.P. 50100.

*Corresponding Author E-mail: jcastilloc@uaemex.mx 

Accepted 12 July, 2017 

Abstract

Exposure to anticancer agents such as cyclophosphamide and adriamycin promote the expression and synthesis of gene products whose function is cell protection, such as the family of isoenzymes of glutathione S-transferase, involved in Phase II detoxification of xenobiotics by glutathione conjugation. Response to the treatment is uncertain and different for each patient. Our objective was to determine total GST and GSTT1 enzyme activity induced by the treatment in women with breast cancer and the variability of the response. In 22 women with breast cancer, the total GST and GSTT1 enzymatic activity before and after treatment. The magnitude of enzymatic activity was different in each patient before and after treatment. The value of the median of total GST enzyme activity before treatment was 2.425 μmol/min/mL and after 3.253 μmol/min/mL, it was significantly different, p<0.05. For GSTT1 the median values of enzymatic activity before and after treatment were 0.015 and 0.021 μmol/min/mL, respectively, it was significantly different, p<0.05. Adriamycin and cyclophosphamide induce the expression of GST isoenzymes by increasing the enzymatic activity after treatment. The heterogeneity of enzymatic activity as a treatment response shall be considered for its prescription.

Keywords: Total GST enzyme activity, GSTT1 enzyme activity, breast cancer, adriamycin, cyclophosphamide

 

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