Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
August 2015 Vol. 4(8), pp. 331-336
Copyright © 2015 Global Advanced Research Journals

 

Full Length Research Paper

PCR Versus Microscopic Examinations for Detection of Mycobacterial tuberculosis in formalin fixed Histologic Specimens

Omar B Ahmed1* and Atif H Asghar1

1Department of Environmental and Health Research, The Custodian of the Two Holy Mosques Institute for Hajj and Umraa. Umm Al-Qura University, Makkah, Saudi Arabia.

*Corresponding   Author   E-mail:   abuaglah1@hotmail.com, Tel:  +966125572855,   Ext: 417 

Accepted 04 August, 2015

Abstract

Diagnosis of tuberculosis (TB) in formalin-fixed, paraffin-embedded tissue specimens depends on microscopic examination (Zeihl-Neelsen stain (ZN-stain), and histopatholgy) but ZN-stain sensitivity is still of primary limitation and histopathological features of chronic granulomatous inflammation can be found in various diseases other than TB. Accurate and early diagnosis of tuberculosis in formalin-fixed, paraffin-embedded tissue samples is important for effective management.  The present study aimed to compare between TB-polymerase chain reaction (PCR), ZN-stain, and histpathological examination for diagnosis of TB in formalin fixed histologic specimens. Out of fifty different tissue specimens clinically diagnosed as TB, ZN-stain was positive in (34%), histological examination was positive in (52%) and TB-PCR was positive in (64%). TB-PCR was the most sensitive (100.0%) with specificity of 90.0% while sensitivity of ZN-stain was the lowest (50%). It was concluded that the PCR assay was more rapid, specific and reliable for TB diagnosis. In addition it should be implemented as routine test in histopathology as well as microbiology laboratories for the diagnosis of TB.

Keywords: Mycobacteria tuberculosis, DNA, Formalin fixed, ZN-stain, PCR.

 

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