Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
August 2016 Issue Vol. 5(8), pp. 221-227
Copyright © 2016 Global Advanced Research Journals

Full Length Research Paper

Antimicrobial activity of nonpolar natural product compounds of Egyptian Ocimum basilicum L

Mohamed H. M. Abd El-Azim*, Amani M. D. El-Mesallamy, Mohamed El-Gerby and Sherin Ali

Department of Chemistry, Faculty of Science, Zagazig University, Egypt.

*Corresponding  Author  E-mail: drmhm1982@gmail.com

Accepted 10 August, 2016

Abstract

In our study; light petroleum ether and diethyl ether extracts of Egyptian Ocimum basilicum L. were analyzed by Gas chromatography-mass spectrometer (GC-MS). Un-saponifiable part of the petroleum ether extract were found to contain twelve compounds with three main constituents were methyl eugenol (87.91%), 4,8,12-Trimethyl-1,3,7,11-tridecatetraene (5.11%) and phytol (3%), and the main constituents of saponifiable fraction of petroleum ether extract contained six fatty acid compounds with two main constituents were α –linoleic methyl ester (65.16 %) and linoleic methyl ester (18.18 %). Also un-saponifiable part of diethyl ether extract were found to contain eight compounds with two main constituents were Cyclopropane,1,1-dimethyl-2-(2,4-pentadienyl) (8.48 %) and 3-Methyl-3-buten-1-ol (5.54%), while saponifiable part gave a traces of free fatty acids. Antimicrobial activities were studied with five bacterial species (Psudomonas aeruginosa, Kelbseilla sp., Salmonella typhi, Staphyllococcus aureus and E. coli, and with five fungal species (Fusarium oxysporum, Aspergillus flavus, Aspergillus niger, Cladosporium sp. and Penicillium sp). Antibacterial activity showed that both extracts have resistance against all bacterial species at 3 mg concentration under investigation. While at 1 mg concentration both extracts have resistance against with Psudomonas aeruginosa, Kelbseilla sp. and E. coli. Also for antifungal activity we found both extracts have resistance against all species 3 mg concentration under investigation. While at 1 mg concentration of both extracts showed no resistance against all species.

Keywords: Antimicrobial activities, GC-MS, Hydrocarbons, Fatty acids and Ocimum basilicum L.