Global Advanced Research Journal of Medicine and Medical Sciences (GARJMMS) ISSN: 2315-5159
September 2014 Vol. 3(9), pp. 267-274
Copyright © 2014 Global Advanced Research Journals

 

Full Length Research Paper

Serotonin levels in plasma and platelets of adolescents with type 1 diabetes

Gabriel Manjarrez-Gutiérrez1,2*, Rocío Herrera-Márquez3, Teresa Neri-Gómez1,  Jorge Hernández-Rodríguez4

1Laboratory of Molecular Pathology, Cardiology Hospital, National Medical Center (CMN-SXXI), Mexican Institute of Social Security (IMSS), Mexico City, Mexico
2Medical Research Unit in Neurological Diseases, Specialties Hospital, CMN-SXXI, IMSS, México, DF, México
3Pediatric Hospital, CMN-SXXI, IMSS, Mexico City, Mexico
4Laboratory of Neurontogeny, Department of Physiology, Biophysics and Neurosciences, Center of Research and Advanced Studies (CINVESTAV) IPN, Mexico City, Mexico.

*Corresponding Author E-mail: willisga@prodigy.net.mx, gmanjarrezg@gmail.com, Phone: (+52) (55) 56276900, Ext. 22156.   

Fax: (+52) (55) 55780240 

Accepted 18 September, 2014

Abstract

The aim of this study was to determine, in adolescents with type 1 diabetes, 5-hydroxytryptamine (5-HT, serotonin) levels in plasma and platelets, 5-HT transporter (SERT), platelet 5-HT2A receptor and transglutaminase 2. We performed a cross-sectional studying two groups: 1) adolescents with type 1 diabetes and 2) controls. Three milliliters of blood was obtained from all patients to determine 5-HT levels in plasma and platelets. Furthermore, platelets were obtained to evaluate SERT expression, 5-HT2A receptor and transglutaminase 2 using Western blot. Dot blot was used for 5-HT. Glucose, glycated hemoglobin, albumin, triglycerides, cholesterol, hemoglobin, mean platelet volume and platelet count were also measured. Results were analyzed using Student t and Mann-Whitney U tests; P < 0.05 was accepted as statistically significant. Adolescents with type 1 diabetes had an elevation of plasma glucose and glycated hemoglobin (P < 0.001) as well as an increase the 5-HT levels in plasma and platelets along with a decreased expression of SERT, 5-HT2A and increased transglutaminase 2 in platelets compared to controls (P < 0.05). Increased the 5-HT levels in plasma and platelets, together with the decreased expression of SERT and 5-HT2A receptors and increase in transglutaminase 2 platelets, may have an important role in the prothrombotic state frequently presented by patients with diabetes.

Keywords: Serotonin, type 1 diabetes mellitus, platelets, SERT, 5-HT2A receptor, transglutaminase 2

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