Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116

June 2014 Vol. 3(5), pp 083-088

Copyright © 2014 Global Advanced Research Journals

Full Length Research Paper

Fecal carriage of extended-spectrum β-lactamase-producing Enterobactearicae: a comparative study between hospitalized and non-hospitalized patients.

Tarek A.A. Moussa¹ Rasha H. ElSherif², Mohamed E.A. Dawoud¹, Reham A. Dwedar³ and Nahla T. Muhammedy¹

¹Botany Department Science Faculty Cairo University

²Clinical Pathology Department, Faculty of Medicine, Cairo University

³Microbiology and Immunology Department, Faculty of Medicine, Cairo University

Corresponding Author’s Email: Rasha.elsherif@kasralainy.edu.eg;

Tel: 02 0100077557; Tel Fax: 0225080099.

Accepted 18 June, 2014

Abstract

Recently the world has seen a surge in extended-spectrum β-lactamase (ESBL)-producing bacteria. However, data on the dissemination of ESBL-producing Enterobacteriaceae in the hospital and in non-hospitalized personal from systematically enrolled study subjects in Egypt remains few.  Aim of study: To determine the prevalence, phenotypic resistance patterns and genetic characteristics of ESBL-producing Entearobacteracicea in fecal carriage in both hospital and in non-hospitalized personal. Material and methods: A total of 160 fecal samples from 60 non hospitalized coming from out patients clinics and100 hospitalized patients .The were screened for the presence of ESBL producing Enterobactearicae. A multiplex PCR assay was used to identify blaCTX-M blaSHV, blaOXA, blaTEM genes. Results: Out of 60 clinical isolates from non-hospitalized patients group (1) it was found that the 41 Escherichia coliisolates produced the different ESBLs types as follow:(58%) produced TEM gene, (95%) produced CTX-M, (54%) produced TEM combined with CTX-M. Out of 100 clinical isolates from hospitalized patients group (II) it was founded that the (50) Escherichia coliisolates produced the different ESBLs types as follow: (78%) produced TEM gene, (96%) produced CTX-M and (74%) produced TEM combined with CTX-M. Conclusion: The most affected age was 15-30 years; this may be due to the panting of this age group on fast food, which may be the reason in the transmission and spread of infection by these microbes. blaCTX-M was the most prevalent ESBL gene followed by blaTEM and finally, blaSHV, while the blaOXA gene was not detected in any of the isolated bacteria.

Keywords: Hospitalized, non hospitalized, ESBL ,fecal.