Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116

June, 2015. Vol. 4(5), pp 060-063

Copyright © 2015 Global Advanced Research Journals

 

Full Length Research Paper

Molecular Cloning and Sequencing of xylanase gene from Penicillium chrysogenum

Chijioke A. Nsofor¹  and Jensen Mueller²

 

¹Department of  Biotechnology, Federal Univeristy of Technology Owerri, Nigeria

²Department of Microbiology and Biotechnology, University of Stuttgart, Germany

*Corresponding Author's Email: nsoforac@gmail.com

Accepted 10 June, 2015

 

Abstract

 

The filamentous fungus, Penicillium chrysogenum, is able to grow on xylan as a sole carbon source. Under these conditions, high levels of a xylanase (XYLP) are secreted into the medium. Xylans are the principle non-starch polysaccharides of wheat, and top surface of wheat in poultry diets can increase the intestinal viscosity, that is obstacle the absorption of nutrients and circulation. In this study, we clonned xylanase gene from Penicillium chrysogenum in pGEM vector and the construct was transformed into TOP10 E. coli cells after purification and characterization of the enzyme. Genomic sequencing was done by using oligodeoxyribonucleotides derived from partial amino acid (aa) sequences of the purified enzyme. Sequencing confirmed that xylanase was succesfuly cloned and the length of xylanase was 661 bp; BLAST search showed that the sequence of xylanase gene of the Penicillium chrysogenum has 97% homology with other records existing in GenBank.  The aa sequence of XYLP shows considerable homology to high-M(r) acidic xylanases (Xln) and cellulases from different bacteria, yeasts and fungi.

Keywords: Xylanase; Gene cloning and expression; Sequence analysis Penicillium chrysogenum.

 

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